Glossary entry (derived from question below)
German term or phrase:
Restriktionsverdau
English translation:
restriction digest
Added to glossary by
nocciola
Dec 14, 2006 13:46
17 yrs ago
3 viewers *
German term
Restriktionsverdau
German to English
Science
Genetics
Listed under "Detection of PCR products" in the description of a molecular biology course. I am translating references/Zeugnisse.
Is this Restriction enzyme digestion?
Your help urgently required!
Thank you, Hazel
Is this Restriction enzyme digestion?
Your help urgently required!
Thank you, Hazel
Proposed translations
(English)
4 +4 | restriction digest | Steffen Walter |
Proposed translations
+4
2 hrs
Selected
restriction digest
See parallel German/English sources
http://www.biol.ethz.ch/IMB/groups/berger_group/Lectures/Pra... (see pp. 3, 4 - Table of Contents, Part B)
http://www.botanik.univie.ac.at/lehre/lehrveranstaltungen/ss...
and native English sources
http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/dig...
http://intranet.bpc.ac.uk/courses/download/bmsi/co/catering/...
"Prepare a separate restriction digest for each presumptive positive PCR amplification, and amplification products from control Cyclospora cayetanensis and Eimeria tenella strains."
http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/pr...
"Stages in protocol
(1) Restriction digest of PAC DNA
(2) ligation of restriction digests
(3) PCR amplification of ligation products
(4) Sequencing of PCR products
(5) Trim sequence of primer and vector sequence"
http://ysp.ucdavis.edu/Research06/HuJ/default.html
"Restriction digest: Specific restriction enzymes (Stu1, Pvu1, Dra1) were added along with their respective buffers to eluted PCR reactions and incubated at 37ºC for two to three hours. Afterwards, gel electrophoresis was conducted as with a regular PCR reaction."
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Note added at 2 hrs (2006-12-14 16:18:43 GMT)
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See also http://www.methodbook.net/dna/restrdig.html and http://preuss.bsd.uchicago.edu/protocols/enzyme.html - the longer version "restriction enzyme digest(ion)" is also used.
http://www.biol.ethz.ch/IMB/groups/berger_group/Lectures/Pra... (see pp. 3, 4 - Table of Contents, Part B)
http://www.botanik.univie.ac.at/lehre/lehrveranstaltungen/ss...
and native English sources
http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/dig...
http://intranet.bpc.ac.uk/courses/download/bmsi/co/catering/...
"Prepare a separate restriction digest for each presumptive positive PCR amplification, and amplification products from control Cyclospora cayetanensis and Eimeria tenella strains."
http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/pr...
"Stages in protocol
(1) Restriction digest of PAC DNA
(2) ligation of restriction digests
(3) PCR amplification of ligation products
(4) Sequencing of PCR products
(5) Trim sequence of primer and vector sequence"
http://ysp.ucdavis.edu/Research06/HuJ/default.html
"Restriction digest: Specific restriction enzymes (Stu1, Pvu1, Dra1) were added along with their respective buffers to eluted PCR reactions and incubated at 37ºC for two to three hours. Afterwards, gel electrophoresis was conducted as with a regular PCR reaction."
--------------------------------------------------
Note added at 2 hrs (2006-12-14 16:18:43 GMT)
--------------------------------------------------
See also http://www.methodbook.net/dna/restrdig.html and http://preuss.bsd.uchicago.edu/protocols/enzyme.html - the longer version "restriction enzyme digest(ion)" is also used.
Note from asker:
Thank you very much for your help and all the background information. Like Mr. Sampat , I was astounded with all the research you did!! Today I joined proz. However, I am afraid that I won't be able to help people like you have helped me because I am fairly new to professional translation! Hazel (Nocciola) |
4 KudoZ points awarded for this answer.
Comment: "Thank you very much for your wonderful support!"
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